Mda Mb 231 Doubling Time

Subculture medium 0 25 w v trypsin 0 53 mm edta atcc cat.

Mda mb 231 doubling time. I am carrying some mda mb 453 cells. However if my doubling time is 24hrs then i will reach 125 confluence on day 3. The mda mb 231 cell line is aepithelial n human breast cancer cell line that was established from a pleural effusion year old caucasian femaleof a 51 with a metastatic mammary adenocarcinoma1 and is one of the most commonly used breast cancer cell lines in medical. Mda mb 231 atcc htb 26 homo sapiens mammary gland breast.

Mda mb 231 ecacc catalogue no. They seem to grow very slowly. Growth properties population doubling time pdt is approximately 47 h see appendix 2. I am using the medium recommended by atcc.

Any trick with growing these cells. The breast cancer cells investigated had wildly different growth rates in culture with doubling times ranging from 18 20 h for the aggressive triple negative mda mb 231 to 80 h for the estrogen sensitive zr75 1 line cf. The reason why mda mb 231 cells could be successfully adapted to the serum free growth medium uc but not to ps might be the different supplement composition of both growth media. Thawing propagation and cryopreservation of nci pbcf htb26 mda mb 231 page 4 of 25 growth properties population doubling time pdt is approximately 38 h.

Special growth requirements avera subculture mda mb 468 cells at 75 to 85 confluence or when cell density reaches an ge of 1 5 x 105 viable cells cm2. Leibovitz s l 15 medium with 2 mm l glutamine 10 fbs. Mda mb 231 cells possess epithelial like morphology and exhibit invasive properties when cultured in vitro and when transplanted into mice. To atcc valued customers atcc stands ready to support our customers needs during the coronavirus pandemic.

Mda mb 231 is a metastatic human breast cancer cell line originally isolated in the early 1970s 1. Growth kinetics such as the doubling time 27 5 hours for uc were comparable to mda mb 231 cells cultivated with 10 serum containing basal medium dmem f 12 28 1 hours. I m looking to do a time course realtime glo vitality assay on mda mb 231 in a 96 well plate. Who can tell me the doubling time for this cell line.