Mda Mb 231 Cells Doubling Time

Xe is the cell number at the end of the incubation time.

Mda mb 231 cells doubling time. Mda mb 231 cells possess epithelial like morphology and exhibit invasive properties when cultured in vitro and when transplanted into mice. Dt t ln2 ln xe xb t is the incubation time in any units. Essentially cells were detached by pipetting. Cell number in vial cells 1ml 3 9 x 10 6.

Mda mb 231 is widely used as a model for. Mda mb 231 is a metastatic human breast cancer cell line originally isolated in the early 1970s 1. Cell micrograph cancer cell line mutation data transfex protocol mda mb 231 cells. The reason why mda mb 231 cells could be successfully adapted to the serum free growth medium uc but not to ps might be the different supplement composition of both growth media.

Some cells in the culture usually. Viability at cell freezing 92 2. Complete growth medium the complete growth medium used to expand mda mb 231 cells is l 15 10 v v fbs. The cells are somewhat spindle shaped long and thin especially at subconfluence.

Some references are seeding at 5k cells per well however if my doubling time is 24hrs then i will reach. Mda mb 231 cells should be seeded at a density between 13 x 10. For mda mb 231 cells cell doubling time was 27 78 5 14 hours and 29 92 2 85 hours measured with the srb assay and the xcelligence system respectively. 2 1 is primarily important because my time course is every 24 hrs for either 3 4 days.

Calculate the population doubling time or the time required for a culture to double in number with the following formula. In case adherent cells still exist use 0 25 trypsin and 0 02 edta to detach the cells. Faq s morphology of atcc htb 26 the atcc htb 26 cell line is epithelial like. Growth kinetics such as the doubling time 27 5 hours for uc were comparable to mda mb 231 cells cultivated with 10 serum containing basal medium dmem f 12 28 1 hours.

Xb is the cell number at the beginning of the incubation time. Similarly for a549 cells doubling time was 27 93 1 75 hours and 29 18 1 87 hours measured with the srb assay and the xcelligence system respectively. And subcultured when 70 80 confluent.