Mda Mb 231 Cell Line Atcc

Atcc was entrusted with its first cell line in 1962 and has consistently attained the highest standards and used the most reliable procedures to verify every cell line since.

Mda mb 231 cell line atcc. Background information on mda mb 231 cell line designations. The base medium for this cell line is atcc formulated leibovitz s l 15 medium catalog no. Cell growth curves the mda mb 231 and mcf7 cells were seeded at a density of 1 5 104 cells in 24 well plates. The mda mb 231 cell line isolated at m d anderson from a pleural effusion of a patient with invasive ductal carcinoma is commonly used to model late stage breast cancer.

Human breast cancer cell line atcc htb 26. To make the complete growth medium add the following components to the base medium. Cell line profile. Endothelial cell growth supplement ecgs 30 g ml and heparin 100 g ml.

Mda mb 231 page 3 of 25 protocol for thawing propagation and cryopreservation of nci pbcf htb26 mda mb 231 atcc htb 26 breast adenocarcinoma 1. This cell line is er pr and e cadherin negative and expresses mutated p53. Frozen in dry ice growth properties. Adherent see appendix 1 organism.

The base medium for this cell line is atcc formulated leibovitz s l 15 medium catalog no. A over expression of the mir 212 132 cluster by mimics suppressed the migration of mda mb 231 and expansion of t47d cells in wound healing assay compared with sins. Every step of the way a. The atcc cell biology collection is one of the largest bioresources in the world and offers a complex array of human animal insect fish and stem cell lines from which to choose.

Cell culture growth conditions transient transfection. Mda mb 231 was isolated from pleural effusions of a caucasian breast cancer patient. All of the cell lines were maintained at 37 c under a humidified 5 co 2 atmosphere. The mda mb 231 cell line is aepithelial n human breast cancer cell line that was established from a pleural effusion year old caucasian femaleof a 51 with a metastatic mammary adenocarcinoma1 and is one of the most commonly used breast cancer cell lines in medical.

The role of mir 212 132 cluster on migration of mda mb 231 expansion of t47d and invasion of breast cancer cells were examined by the transfection of mirna mimics mir 132 and mir 212 or antisense as mir 132 as mir 212. Cell lines m mda mb 231 cells. In microarray profiling the mda mb 231 cell genome clusters with the basal subtype of breast cancer. Atcc complete growth medium.

Faq s morphology of atcc htb 26 the atcc htb 26 cell line is. To make the complete growth medium add the following components to the base medium.